Salivary flow rate exerts an essential impact on the development and progression of dental erosion. In this work, the experimental dental erosion in non-obese diabetic (NOD) mice with reduced salivary flow rate was induced, and the erosive effect of acidic drinks on their dentition was studied.
Material and methods:
Three acidic drinks (sports drink, cola light drink and sugar containing cola drink) were given to adult NOD mice (groups: N=11) as the only drink for 6 weeks. Two control groups were included; wild type and NOD control (groups: N=9). Experimental and control (water) teeth were dissected out and observed by scanning electron microscopy (SEM). Mandibular first molars were subsequently embedded in Epon, ground transversely, observed again by SEM, and the enamel thickness and tooth height were measured.
Mandibular molars were considerably more eroded than maxillary molars. The erosive process started at the top of the cusps and subsequently extended in the cervical, mesio-distal, and pulpal direction. Erosive lesions were evident in increased succession from sports drink, cola light to cola drink exposed mandibular molars, with the lingual tooth height being approximately 23%, 26%, and 37% lower, respectively, compared to the control. The lingual enamel was approximately 48% thinner in sports drink molars and 62% thinner in cola light molars. In cola drink molars, the lingual enamel was totally eroded, and significant erosion of dentine was evident.
Reduced salivary flow, together with a high consumption of acidic drinks, results in severe erosion of NOD mice molars.

Dental erosion in mice with impaired salivary gland function
Tulek A, Mulic A, Stenhagen KR, Galtung HK, Saeed M, Utheim TP, Khuu C, Galteland P, Sehic, A
Acta Odontologica Scandinavica, Volum 78, 2020 pages 390-400